@article{Ramy F. Ghobrial_Samar M. Atwa_Mohamed el Beskawy_Verginia M. Farag_Mohamed Eisa_Doaa Naguib_Mohamed EL-Diasty_2023, title={Comparative Immune Responses and Cytokine Gene Expressions in Sheep Vaccinated with Brucella abortus RB51 Vaccine and Brucella melitensis Rev. 1 Vaccine}, volume={13}, url={https://advetresearch.com/index.php/AVR/article/view/1110}, abstractNote={<p> </p> <p>This work applied three <em>Brucella</em> vaccination protocols in adult <em>Brucella</em>-free ewes. Serum and blood samples were collected from each group at time points 0, 7, 14, 21, 28, and 60 days post-vaccination. In addition, the humoral immune response was assessed by the Rose Bengal Plate test (RBPT) every week for 16 weeks. Also, cell-mediated immunity was evaluated. Additionally, interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF- α) were assessed. The results indicated that the cellular immune responses induced by the <em>B. abortus</em> RB51 vaccine with a dose of 3.4 x10<sup>10</sup> provide a protective immune response near the effect produced by<em> the B. melitensis</em> Rev. 1 vaccine. Moreover, the IL-6 was expressed significantly less in the RB51- vaccinated group with a dose of 1.7 x10<sup>10 </sup>CFU (1 ml subcutaneously), greater in the Rev. 1-vaccinated group with a dose of 1.6 × 10<sup>9</sup> CFU (1 ml subcutaneously), and highest expression in the RB51-vaccinated group with a dose of 3.4 x10<sup>10</sup> CFU ( 2 ml subcutaneously). Also, the expression of TNF- α was lowest in the group vaccinated with RB51 vaccine with a dose of 1.7 x10<sup>10</sup> CFU, with greater abundance in the group vaccinated with Rev. 1 vaccine and highest expression in the group vaccinated with RB51 vaccine with a dose of 3.4 x10<sup>10</sup> CFU. These findings imply that the RB51 vaccine, given to sheep with a dose of 3.4 x 10<sup>10</sup> CFU, may trigger protective immune responses and be applied in the field to control brucellosis in sheep.</p>}, number={1}, journal={Journal of Advanced Veterinary Research}, author={Ramy F. Ghobrial and Samar M. Atwa and Mohamed el Beskawy and Verginia M. Farag and Mohamed Eisa and Doaa Naguib and Mohamed EL-Diasty}, year={2023}, month={Jan.}, pages={1-8} }