@article{Mubarak_El-Zamkan_2019, title={Evaluation of Some Virulence Factors of Methicillin and Vancomycin Resistant Staphylococcus epidermidis Isolated from Cheese and Human Samples}, volume={9}, url={https://advetresearch.com/index.php/AVR/article/view/404}, abstractNote={<p>Biofilm formation and enterotoxin production represent major virulence factors of <em>S. epidermidis</em>. Also, biofilm formation is greatly associated with multidrug resistance. So the objective of the present study was to expand the current knowledge regarding the importance and virulence of methicillin and vancomycin resistant <em>S. epidermidis</em> originated from dairy food, food handlers and patients in a hospital, and highlight the possible transmission through foods and food handlers. Biofilm formation was evaluated phenotypically by the tube method and microtiter plate method and genotypically through detection of <em>icaA</em> and <em>icaD</em> genes, while <em>S. epidermidis</em> isolates were investigated for potential enterotoxin production through detection of enterotoxin encoding genes (<em>sea</em>, <em>seb</em> and <em>sec</em>). Among the investigated isolates, phenotypic and genotypic biofilm formation was confirmed in 78.4 and 66.7 % of the isolates, respectively. Regarding enterotoxin encoding genes, it was found that seb gene was the only prevailing gene in the three categories of samples with an incidence of 27.5 %. The findings of this study illustrated the prominent role that played by food handlers in transmission of virulent <em>S. epidermidis</em> to food and subsequently patients.</p>}, number={4}, journal={Journal of Advanced Veterinary Research}, author={Mubarak, Asmaa Gaber and El-Zamkan, Mona Ahmed}, year={2019}, month={Oct.}, pages={187-196} }