@article{Abo El-Maaty_Abdelhafez_Mahrous_2022, title={Effect of Diet Restriction and Polymorphism of Bone Morphogenetic Protein-15 and Growth Differentiation Factor-9 (GDF9) on Reproductive Performance of Three Egyptian Fat Tail Sheep Breeds}, volume={12}, url={https://advetresearch.com/index.php/AVR/article/view/829}, abstractNote={<p>Several genes are controlling prolificay of sheep. The bone morphogenetic protein-15 and growth differentiation factor-9 (GDF9) are control reproductivity of sheep. This study aimed to eplore diet restriction and the polymorphism in the bone morphogenetic protein-15 (<em>BMP</em>15) and growth differentiation factor-9 (<em>GDF</em>9) genes on ovulation and reproductive hormones in three sheep breeds. Ovaries of Rahmani ewes (R, <em>n</em>=56), Barki (B, <em>n</em>=56), and Ossimi ewes (O, <em>n</em>=50) were scanned to determine the preovulatory follicles and ovulation. The harvested sera were used for assaying progesterone, leptin, insulin-like growth Factor-I, and insulin. Whole blood samples were used for gene analysis using two PCR primers for amplifying the fragment 141-bp for <em>Fec</em>XG site (exon-2) of <em>the BMP</em>15 gene and 139-bp for <em>Fec</em>GH locus (exon-1) of <em>the GDF</em>9 gene. The output amplicons were digested (RFLP) with <em>Hinf</em>I and <em>Dde</em>I endonucleases. Results revealed no mutation in <em>the Fec</em>XG locus in all breeds. <em>Fec</em>GH showed mutations but not in all breeds. Treated O received half dietary requirements for four weeks were the youngest (<em>P=</em>0.0001). Barki had the lightest bodyweight (<em>P=</em>0.017). Treated ewes had a higher (<em>P=</em>0.047) number of large follicles compared to their controls. Treated B and R got larger (<em>P=</em>0.0001) dominant follicles. The ovulation rate did not vary within the diet treated breeds but was lower than their control. Control O and R had higher (<em>P=</em>0.0001) ovulation rate, compared to B. Leptin concentrations were low (<em>P=</em>0.047) in treated B and R. The lowest (<em>P=</em>0.031) insulin concentrations were observed in treated O. In conclusion, the <em>BMP</em>15 loci showed no polymorphism, while the <em>GDF</em>9 loci were polymorphic in all breeds and treatments. Nutritional status and age modified ovulation rate in sheep.</p>}, number={1}, journal={Journal of Advanced Veterinary Research}, author={Abo El-Maaty, Amal Mahmoud and Abdelhafez, Mohamed A. and Mahrous, Karima F.}, year={2022}, month={Jan.}, pages={18-24} }