@article{Badr_Roshdy_Kilany_Elfeil_Sedik_Mohammed_Shalaby_2022, title={Isolation and Molecular Identification of Avibacterium paragallinarum in Suspected Cases of Poultry}, volume={12}, url={https://advetresearch.com/index.php/AVR/article/view/960}, abstractNote={<p>Infectious Coryza (IC) is an infectious upper respiratory disease of chickens and birds, caused by <em>Avibacterium paragallinarum (Av. Paragallinarum</em>). It is characterized by facial swelling and nasal discharge. Here, the study aimed to identify the causative agent of infectious coryza from diseased cases using conventional and molecular methods, forty-one different samples (swollen heads and nasal discharges) from layers and broiler chickens were examined bacteriologically for isolation of <em>Av. Paragallinarum</em>.  The total isolation percentage was 9.75%, and the percentage was 66.7% from total layer samples. The samples underwent examination by polymerase chain reaction (PCR) using HPG-2. Three samples were confirmed by PCR with a percentage of 7.3% (3/41). Multiplex PCR was used for typing the tested strains using HMTp210 gene thus, all strains were of type B. sequence analysis showed a maximum identity percentage (100%) between the tested strains of <em>Av. paragallinarum</em>. In conclusion, typical signs for IC diseases were appeared and identified bacteriologically as <em>Av. paragallinarum</em> also three strains were only confirmed by PCR and identified type B strains. PCR technique is a reliable and sensitive test for confirmation<em>. </em></p>}, number={3}, journal={Journal of Advanced Veterinary Research}, author={Badr, Heba and Roshdy, Heba and Kilany, Walid and Elfeil, Wael and Sedik, Ahmed and Mohammed, Wafaa and Shalaby, Azhar}, year={2022}, month={Jun.}, pages={253-258} }