TY - JOUR AU - Rouby, Sherin R. AU - Abdel-Latef, Gihan K. AU - Abdel Aziz, Sahar Abdel Aleem PY - 2019/07/03 Y2 - 2024/03/28 TI - Bacteriological and Molecular Identification of Thermophilic Campylobacters of Animal and Human Origins in Beni-Suef Governorate, Egypt JF - Journal of Advanced Veterinary Research JA - JAVR VL - 9 IS - 3 SE - DO - UR - https://advetresearch.com/index.php/AVR/article/view/377 SP - 102-106 AB - <p>Thermophilic species of the genus <em>Campylobacter</em> are generally considered commensals of livestock and the leading cause of bacterial food-borne zoonoses. The present study was delineated to clarify the role of <em>Campylobacter</em> species as a diarrheagenic pathogen in animals and man and to investigate the fecal carriage rate of <em>Campylobacters </em>in animals and in-contact humans. A total number of 78 fecal samples were collected from diarrheic and non-diarrheic cattle (n=26), sheep (n=28) and humans (n=24). Samples were enriched in Preston broth, followed by streaking on selective <em>Campylobacter</em> agar base medium. The suspected colonies were tested morphologically and biochemically. <em>Campylobacter</em> spp. was recovered from 29 (37.17%) out of 78 fecal samples (34.61%, 42.85% and 33.33%) for cattle, sheep and humans, respectively. Positive correlation between the occurrence of diarrhea and the isolation of <em>Campylobacters</em> was observed in samples of human origin while in adult ruminants particularly sheep, high fecal carriage rate was observed in non-diarrheic animals. The isolates were identified to genus and species levels by polymerase chain reaction targeting the 16S <em>rRNA</em> gene, the <em>mapA</em> gene and the <em>ceuE</em> gene which revealed that all of isolates were <em>Campylobacter jejuni</em>. These findings pose a significant epidemiological implication where cattle and sheep act as vehicles of, and excrete <em>Campylobacter jejuni</em> which is capable of causing disease in the local community in the area of investigation.</p> ER -