TY - JOUR AU - Saleh, Shimaa AU - Salam, Hala AU - Hassan, Abdelrahim PY - 2021/10/01 Y2 - 2024/03/29 TI - Antibacterial Activity of Origanum majorana and Curcuma longa Extracts against Multiple Drug-resistant Pathogenic E. coli and Methicillin-resistant Staphylococcus aureus Isolates Recovered from Meat Products JF - Journal of Advanced Veterinary Research JA - JAVR VL - 11 IS - 4 SE - DO - UR - https://advetresearch.com/index.php/AVR/article/view/787 SP - 221-229 AB - <p>The presence of antimicrobial-resistant foodborne pathogens in our food can threaten our life. Thus, great attention was paid to find a potentially effective, safe, and natural antimicrobial agent. Therefore, this study carried out this study to in-vitro investigate the potential use of Origanum majorana and Curcuma longa as natural antimicrobial food additives to control multidrug-resistant foodborne pathogens isolated from minced beef and beef burger samples. Herein, we examined 100 raw meat product samples, i.e., 50 each of fresh minced beef and frozen beef burger samples, randomly collected from butcher’s shops in Egypt, for the presence of pathogenic <em>E. coli</em> and <em>S. aureus</em>. Pathogenic <em>E. coli</em> was detected in 36 % of examined minced beef samples, while we failed to isolate it from beef burger. On the other hand, coagulase-positive <em>S. aureus</em> was found in 26 and 10 % of minced beef and beef burger samples, respectively. The multiple-drug resistant (MDR) isolates of <em>E. coli</em> and <em>S. aureus</em> were identified using 11 and 6 commercial antimicrobial discs, respectively. MDR isolates were selected for molecular identification based on virulence and anti-microbial resistance genes. Molecularly, <em>eae</em>A gene was detected in 100% of identified pathogenic <em>E. coli </em>strains, while <em>stx</em>1 was detected in one strain only. Whereas <em>mec</em>A and <em>coa</em> were detected in 100% of coagulase-positive <em>S. aureus </em>isolates, The antimicrobial effectiveness of Origanum majorana (OM) and Curcuma longa (CL) ethanolic extracts against isolated MDR pathogens were evaluated. OM and CL were potentially effective against MDR coagulase-positive <em>S. aureus </em>with variable inhibition zones ranged from 2mm to25mm. While they did not inhibit pathogenic <em>E. coli</em> strains (O158, O157, O114, O142, O44, O86, O25). Extracts of OM and CL were proved to be potentially effective against MDR coagulase-positive <em>S. aureus, and </em>can be used as a natural alternative food preservative to control <em>S. aureus</em> growth in food in place of chemical antimicrobial agents.</p> ER -