Journal of Advanced Veterinary Research

Impact of Using Emulsifier with Different Calcium and Soybean Oil Levels on Broilers Performance and Minerals Deposition

Wageh Darwish

This study aimed to evaluate the effect of emulsifiers with different calcium and soybean oil levels on Ross 308 broiler performance, digestibility, carcass quality traits, and deposition of calcium, phosphorus, and manganese on the body and tibia of broilers. A total of 360 one-day-old chicks were randomly distributed into 24 treatment groups: three calcium to phosphorus ratios were used, 2:1, 1.8:1, and 1.6:1, and each ratio was divided into two positive control treatments with an emulsifier (Lipidol) and a negative control without an emulsifier; moreover, each treatment used four levels of soybean oil: 1%, 1.5%, 2%, and 2.5%. The results showed that emulsifier supplementation, low calcium levels (1.8:1 and 1.6:1), and high oil levels (2% and 2.5%) significantly elevated (P < 0.05) body weight and body weight gain and reduced (P < 0.05) feed intake and feed conversion ratio in all stages, except for body weight gain and feed conversion ratio during the finisher period, which showed a nonsignificant difference. Moreover, broilers fed the diet with emulsifier had higher dressing and abdominal fat percentage (P < 0.0001) and those fed low Ca level (1.6:1) significantly (P < 0.0001) showed the highest abdominal fat percentage. It can be concluded that an emulsifier with a low oil level could improve fat digestibility in broiler chickens. Furthermore, decreasing the level of calcium at a rate of 1.6:1 can improve fat digestibility and elevate (P < 0.0001) the level of cholesterol on day 10; however, on days 23 and 42, cholesterol level was elevated by high calcium level (2:1). Emulsifier also elevates the cholesterol level at days 10 and 42. Broilers fed emulsifier, low oil level (1%), and high calcium levels at rates of 1.8:1 and 2:1 significantly (P < 0.05) had elevating levels of calcium and phosphorus and decreased manganese levels on the broiler body and tibia

Assessment of microbial safety and quality of market raw milk and pasteurized milk sold in Dakahlia governorate, Egypt

Wageh Darwish

In this study, assessing the microbial quality and safety of the market raw milk and pasteurized milk is the basal objective of this study. One hundred and twenty (120) milk samples (60 of each) were collected randomly from different supermarkets and retailer shops in Dakahlia governorate, Egypt. Samples were analyzed for Total bacterial count(TBC), total coliforms(TCC), Total Staphylococcus count (TSC), and Total yeast and mould count. All market milk samples and pasteurized milk were found to be contaminated and judged poor. Listeria spp. were isolated on Oxford agar and then subjected to biochemical and molecular identification. The overall isolation rate of Listeria spp. from the market raw milk was 28.33%. The prevalence rates of Listeria monocytogenes were 13.33% in raw market milk. The prevalence of Listeria innocua and other Listeria species were 3.33% and 11.67%, respectively. Polymerase chain reaction (PCR) was used to detect the virulence genes (16SrRNA, hylA, and prfA) of 8 biochemically identified L. monocytogenes strains recovered from raw market milk using specific primers. 16SrRNA, hylA, and prfA genes are considered the best indicator for virulence determination of L. monocytogenes isolated from market raw milk. A high microbial load of market milk and pasteurized milk may present a public health hazard to the consumers and emphasizes the need for improved hygienic standards.

Caseous Abscess in a Shingleback Lizard (Tiliqua rugosa) with Klebsiella sp. Infection

Puveanthan Nagappan Govendan

An adult male shingleback lizard (Tiliqua rugosa), weighing 700 grams was presented with a swelling growth on the left lobe of the head. Clinical examination indicated a mass growth and the lizard was diagnosed with caseous abscess accumulation. Surgical intervention was performed to remove the caseous abscess. Post-surgery treatment consists of enrofloxacin, meloxicam, tramadol, multivitamin, and topical antibiotic cream. Laboratory bacterial culture from the removed caseous abscess revealed Klebsiella sp. infection. Six months after the surgery the shingleback lizard was presented with no swelling reoccurrence in a clinical healthy condition.

Molecular, epidemiological, and clinical investigations of Anaplasma marginale infection in cattle at Qena governorate, Upper Egypt

abdalrahman ahmed

Bovine anaplasmosis is one of the most important diseases that threaten livestock production worldwide especially in developing countries, in cattle mainly caused by obligate intra-erythrocytic Anaplasma marginale. A. marginale is transmitted biologically by ticks (Hyalomma, Rhipicephalus). Bovine anaplasmosis causes mild to severe clinical signs ranging from anorexia, fever, anemia, and respiratory manifestations to icterus and death. Molecular detection is the best method for Anaplasma diagnosis because of its ability to detect sub-clinical and carrier hosts. This study investigated the occurrence of A. marginale infection among cattle in the Qena governorate utilizing a molecular assay based the msp5 gene. A total of 100 whole blood samples were collected randomly from apparently healthy and diseased cattle. Such cattle were examined clinically and their samples were included for microscopic examination. PCR screening of the tested cattle showed 23% (23/100) as a positive rate. While 6 samples from 100 (6%) showed A. marginale parasite in the microscopic examination. Several risk factors were analyzed in the current study, higher incidence rates were detected in animals less than 2 years than older than 2 years, Holstein-Friesian breeds than cross-breeds and in animals kept in small farms than in the mass farming system. Clinical and hematological variables were also investigated in several infected and non-infected cattle based on PCR reactivity. Fever, anorexia, respiratory manifestations, enlarged lymph nodes, pale or icteric mucous membranes and digestive disorders were reported in infected cattle (n= 23) but not in non-infected animals (n=77). Consistently, hematological variables in infected cattle (n = 10) revealed significantly lower RBCs count and hemoglobin content than those in the non-infected group (n=20) indicating hemolytic anemia. This study shows the high prevalence of A. marginale in cattle in Qena governorate associated with health hazards and multi-risk factors, so frequent usage of acaricides, regular examination of cattle, and successful chemoprophylaxis are recommended.

Response of broiler chickens to the dietary fortification of bile acid

Ahmed Hussien

The feeding trial was conducted for 31 days to investigate whether dietary energy modifications using bile acid feed additive (Runeon®) affected broiler performance, carcass characteristics, blood indices, intestinal lipase activity, and broiler's meat quality. A total of 1200 one-day-old Ross-308 broiler chicks (as hatch) were randomly distributed into three groups, each with five replicates (80 chicks/replicate). The first group was a control (T1) which fed a basal diet only. In the second group (T2), birds were fed the basal diet supplemented with bile acid (Runeon®) (on top application) at the rate of 200g/ton. In the third group (T3), birds were fed a basal diet reduced in energy requirements by 30kcal/kg and reformulated with 200g/ton of bile acid (Runeon®). Birds' diets fortified with bile acid in (T2) or (T3) significantly (P≤0.05) improved body weight, body weight gain, feed conversion ratio (FCR), and European Production Efficiency Factor (EPEF) as compared to the control. The dressing%, breast, thigh, and drumstick yields were improved in T2 and T3 than in control. Supplementation of bile acid significantly (P≤0.05) reduced abdominal fat%, as well, blood cholesterol, triacylglycerol, HDL, and LDL concentrations, but increased total protein concentration (P≤0.05). Additionally, intestinal lipase levels significantly (P≤0.05) increased in groups fortified with bile acid (T2 and T3). Besides, chicken meat moisture% and fat% were significantly (P≤0.05) decreased in T3 compared to T1 and T2. Conclusively, dietary fortification of bile acid could improve growth performance, profitability, carcass traits, serum lipids profile, intestinal lipase secretion, and chicken meat quality in broiler chickens.