Coccidiosis and Hemorrhagic Necrotic Typhlitis in Broilers Fed without Antibiotic Growth Promoter

Abstract

This study aims to determine the level of damage and the inflammatory response in the cecal epithelium of broiler chickens infected with Eimeria spp. The sample population consists of 39 broiler cadavers from Tabanan and Bangli Regencies, Bali, Indonesia, which showed clinical signs of weakness, drooping wings, and bloody stools before death. Subsequently, they were necropsied, and the swollen cecum tissue, with a color range of pink to black, was sufficiently harvested and placed in a pot filled with 10% neutral buffer formalin. After fixation for 24-48 hours, the sample was then processed routinely and stained with hematoxylin-eosin (HE) dyes. The histopathological examination revealed the occurrence of bleeding, epithelial desquamation, gland necrosis, macrophage infiltration, and various stages of Eimeria spp. development. The presence of E. tenella in the epithelium of the tissue was assumed to be associated with hemorrhage necrotic typhlitis.