A Limited Sero-Surveillance of Foot and Mouth Disease in Sheep in Egypt
Keywords:FMD; SNT; ELISA; PrioCHECK; virus isolation; FMDV serotypes O1&A, Egypt; sheep
The present work was carried out to evaluate the current status of Foot and Mouth Disease (FMD) in sheep in Egypt. Blood-sera of sheep (N.= 100) located in different areas of 5 Governorates; Menofia, Giza, Fayoum, Beni-Sueif and Menia, were collected and serologically tested during 2010. Fifty samples of saliva and oesopharyngeal fluid were collected and examined virologically. Serum neutralization test and enzyme linked immunosorbant assay (ELISA) indicated that 16 % of the tested sera were positive to FMD-antibodies against type O1 and A. PrioCHECK blocking ELISA was achieved and indicated that 100% of detected FMD antibodies were due to infection. Trials of virus isolation in baby mice and calf kidney cell culture indicated that both types of FMD virus (O1 & A) are existed and persisted in Egyptian sheep. It could be recommended that great importance should be practiced of sheep vaccination with the local bivalent FMD vaccine to restrict the spread of infection and to control FMD.
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