Phenotypic and Genotypic Characteristics of Antimicrobial Resistance of Gram-negative Bacteria isolated From Pet Animal

Abstract

Most animal feeds are set from protein-rich raw materials. These protein constituents may possess various hazards, particularly highly drug-resistant pathogens, causing a bad impact not only on the pet's health, but also on their owners. In the current study, a total of 2100 pet food and 100 pets’ fecal swabs were collected and bacteriologically examined from 2017 to 2020. It was revealed that the percentage of Gram-negative bacteria isolated from pet food and fecal swabs was 49% and 56% respectively. E. coli, Proteus sp., and K. pneumoniae were the most isolated bacteria in percentages of 12.4%, 8.4%, and 4.9% respectively from Pet food and 25%, 7%, 12% respectively from pet fecal swabs. In addition, Enterobacter cloacae, P. aeruginosa, Aeromonas hydrophila, Citrobacter sp., P. fluorecens, and Y. enterocolitica were isolated from pet food in order to 3.8%, 3.5%, 3.2%, 2.6%, 2.6% and 2.1% respectively. Salmonella sp. isolated from pet food was 0.6% while it was 5% from pet fecal swabs. The most predominant salmonella serotype isolated from pet food and pet fecal swabs was S. Typhimurium. Furthermore, S. Virchow, S. Anatum, S. Kentucky, S. Kedougou and S. Infantis were isolated serotypes from Pet food in percentages of 15.7%, 23.1%, 15.4%, 7.7%, and 7.7% respectively. While S. Nitra, S. Ibargi, S. Enteritidis and S. Boecker were isolated from pet fecal swabs at a percentage of 20% for each. On the other hand, O158 was the most predominant E. coli serogroup isolated from pet food and pet fecal swabs in percentages of 30.4% and 30.8% respectively followed by O157 in percentages of 21.7% and 26.9% respectively. O26 was isolated from pet food and pet fecal swabs in percentages of 13% and 7.7% for each. O119 was isolated from pet food and pet fecal swabs in percentages of 4.3% and 3.8% respectively. O86, O27, O44, O55, and O78 were isolated from pet food in the percentage of 4.3%, 8.7%, 4.3%, 4.3%, and 8.7%respectively. While O114, O111, and O125 were isolated serotypes from pet fecal swabs in percentages of 15.4%, 3.8%, and 11.5% respectively. This study revealed that the antimicrobial sensitivity test of 80% of Salmonellae were resistant to Cefotaxime and Colistin sulphate while 50%, 30, and 20% of isolates were resistant to Gentamicin, Tetracycline, and Cefepime respectively, while 40% of Salmonellae were resistant to Chloramphenicol, Enrofloxacin, and Amoxicillin-clavulanate. Also 60% of Salmonellae showed resistance to Trimethoprim sulfamethoxazole and Ciprofloxacin. Detection of Extended-spectrum ß-lactamase resistance genes (bla_TEM, bla_SHV, and bla_CTX-M) in Pets using Polymerase chain reaction (PCR) showed the presence of bla_TEM and bla_SHV genes in all tested isolates in 12 samples out of 12 (100%) and has shown that the ratio of bla_CTX-M is 5 out of 12samples (41.6 %).