Comparative Immune Responses and Cytokine Gene Expressions in Sheep Vaccinated with Brucella abortus RB51 Vaccine and Brucella melitensis Rev. 1 Vaccine

Authors

  • Ramy F. Ghobrial Agricultural Research Center (ARC), Animal Health Research Institute-Mansoura provincial Lab (AHRI-Mansoura) P.O. Box 264-Giza, Cairo 12618, Egypt.
  • Samar M. Atwa Department of Internal Medicine, Infectious and fish diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
  • Mohamed el Beskawy Department of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University P.O. Box 51744-Matrouh.
  • Verginia M. Farag Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
  • Mohamed Eisa Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 445, Egypt.
  • Doaa Naguib Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt19, Egypt.
  • Mohamed EL-Diasty Agricultural Research Center (ARC), Animal Health Research Institute-Mansoura provincial Lab (AHRI-Mansoura) P.O. Box 264-Giza, Cairo 12618, Egypt.

Abstract

 

This work applied three Brucella vaccination protocols in adult Brucella-free ewes. Serum and blood samples were collected from each group at time points 0, 7, 14, 21, 28, and 60 days post-vaccination. In addition, the humoral immune response was assessed by the Rose Bengal Plate test (RBPT) every week for 16 weeks. Also, cell-mediated immunity was evaluated. Additionally, interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF- α) were assessed. The results indicated that the cellular immune responses induced by the B. abortus RB51 vaccine with a dose of 3.4 x1010 provide a protective immune response near the effect produced by the B. melitensis Rev. 1 vaccine. Moreover, the IL-6 was expressed significantly less in the RB51- vaccinated group with a dose of 1.7 x1010 CFU (1 ml subcutaneously), greater in the Rev. 1-vaccinated group with a dose of 1.6 × 109 CFU (1 ml subcutaneously), and highest expression in the RB51-vaccinated group with a dose of 3.4 x1010 CFU ( 2 ml subcutaneously). Also, the expression of TNF- α was lowest in the group vaccinated with RB51 vaccine with a dose of 1.7 x1010 CFU, with greater abundance in the group vaccinated with Rev. 1 vaccine and highest expression in the group vaccinated with RB51 vaccine with a dose of 3.4 x1010 CFU. These findings imply that the RB51 vaccine, given to sheep with a dose of 3.4 x 1010 CFU, may trigger protective immune responses and be applied in the field to control brucellosis in sheep.

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Published

2023-01-02

How to Cite

Ramy F. Ghobrial, Samar M. Atwa, Mohamed el Beskawy, Verginia M. Farag, Mohamed Eisa, Doaa Naguib, & Mohamed EL-Diasty. (2023). Comparative Immune Responses and Cytokine Gene Expressions in Sheep Vaccinated with Brucella abortus RB51 Vaccine and Brucella melitensis Rev. 1 Vaccine. Journal of Advanced Veterinary Research, 13(1), 1-8. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1110

Issue

Vol. 13 No. 1 (2023): January 2023

Section

Original Research

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license