Effect of Melatonin-loaded Chitosan Nanoparticles (CMN) on Gene Expression of In-vitro Matured Buffalo Oocyte

Authors

  • Hossam N. Tawfik Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute National Research Centre, Egypt.
  • Omaima M. Kandil Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute National Research Centre, Egypt.
  • Ismail M. Ahmad Department of Biochemistry and Molecular biology, Faculty of veterinary medicine, Cairo University, Giza, Egypt.
  • Maha Mansour Department of Biochemistry and Molecular biology, Faculty of veterinary medicine, Cairo University, Giza, Egypt.
  • Hazem A. El-Debaky Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute National Research Centre, Egypt.
  • Korany A. Ali Center of Excellence for Advanced Science, Advanced Materials and Nanotechnology Group, Applied Organic Chemistry Department, National Research Centre, Egypt.
  • Esraa A.S. Ismail Department of Animal Reproduction & Artificial Insemination, Veterinary Research Institute National Research Centre, Egypt.
  • Samy A. Abedelaziz Department of Biochemistry and Molecular biology, Faculty of veterinary medicine, Cairo University, Giza, Egypt.

Keywords:

Buffalo oocyte , Melatonin-loaded chitosan nanoparticle (CMN) , In-vitro maturation , Relative gene expression, Melatonin

Abstract

This study aimed to evaluate the effect of melatonin and melatonin-loaded chitosan nanoparticle (CMN) supplementation to maturation media on buffalo oocyte maturation rate and relative expression of genes: growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), B Cell Lymphoma 2 (BCL2), Associated X protein (BAX) and superoxide dismutase 1 (SOD1). Buffalo ovaries were heaved from Al-Mounib slaughterhouse, cumulus-oocyte complexes (COCs) were in vitro matured in three different media, TCM-199 medium (control), TCM-199 with melatonin 10-9M, and TCM-199 with CMN 10-9M. The assessment of the nuclear maturation rate was carried out through the presence of the first polar body. In addition, the mature buffalo oocytes were stored on RNA later for genetic analysis of GDF9, BMP15, SOD1, BCL2, and BAX genes using quantitative real-time PCR (qRT-PCR). The results were reported that buffalo oocytes supplemented with melatonin-loaded chitosan nanoparticle (CMN) or melatonin have a significant effect on nuclear maturation rate 94.04±0.65 and 88.74±0.77 respectively when compared with buffalo oocytes matured with basic media (control) 79.67±1.35. Furthermore, buffalo oocytes supplemented with melatonin-loaded chitosan nanoparticle (CMN) or melatonin showed significant upregulation of GDF9, BMP15, SOD1, and BCL2 genes and significant downregulation of BAX gene when compared with oocyte matured with basic media (control). In conclusion, the results of nuclear maturation rate and relative expression pattern of GDF9, BMP15, SOD1, BCL2, and BAX reflect that melatonin-loaded chitosan nanoparticle (CMN) and melatonin` may play an important role in the buffalo oocytes developmental competence.

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Published

2023-06-17

How to Cite

Tawfik, H. N., Kandil, O. M., Ahmad, I. M., Mansour, M. ., El-Debaky, H. A., Ali, K. A. ., Ismail, E. A., & Abedelaziz, S. A. (2023). Effect of Melatonin-loaded Chitosan Nanoparticles (CMN) on Gene Expression of In-vitro Matured Buffalo Oocyte . Journal of Advanced Veterinary Research, 13(4), 656-663. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1408

Issue

Vol. 13 No. 4 (2023): Special Issue: The 6th Scientific Conference on Food Safety (Human and Food Hygiene), Food Safety, Hygiene & Technology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt (3/5/2023).

Section

Original Research

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Copyright (c) 2023 Journal of Advanced Veterinary Research

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