Prevalence and virulence factor genes of Bacillus cereus isolated from milk and some dairy products

Authors

  • Rania M. Ewida Department of Food Hygiene, Safety and Technology (Milk Hygiene), Faculty of Veterinary Medicine, New Valley University, Egypt.
  • Al Shimaa M. Abd EL Mgeed Department of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, Assiut University, Egypt.
  • Tawfik A. El-Bassiony Department of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, Assiut University, Egypt.

Keywords:

Bacillus cereus, Marketable milk,, Dairy products, Virulence genes, Proteolysis, Lipolysis

Abstract

Two hundred and fifty samples (Marketable milk, Ras cheese, Domiati cheese and ice cream), were randomly obtained from various dairy shops, supermarkets and ice cream shops in Assiut City, Egypt, during the period of November 2022 to March 2023. These samples were examined for the detection and counting of Bacillus cereus. The positive B. cereus isolates were analyzed by PCR to detect 16s rRNA gene for B. cereus, cytotoxin K (cyt K) and emetic gene (ces). Out of the 250 examined samples, 14 (5.6%) were contaminated by B. cereus. The analyzed ice cream had the highest prevalence (11.53%), followed by Ras cheese samples. The prevalence of ces gene recorded in the examined isolates was 57.14%, while cyt K gene was found in all isolates. Moreover, proteolytic and lipolytic activity investigations revealed that nearly all strains released protease enzyme (92.85%), most of which (64.28%) could produce lipase enzyme.

Downloads

Published

2023-12-31

How to Cite

Ewida, R. M., Abd EL Mgeed , A. S. M., & El-Bassiony, T. A. . (2023). Prevalence and virulence factor genes of Bacillus cereus isolated from milk and some dairy products. Journal of Advanced Veterinary Research, 14(1), 44-47. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1574

Issue

Vol. 14 No. 1 (2024): January

Section

Original Research

License

Copyright (c) 2023 Journal of Advanced Veterinary Research

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles under the following conditions: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0).

For more information: 

Attribution-NonCommercial-NoDerivs 
CC BY-NC-ND

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license