Effect of Seminal Plasma Removal on Cell Membrane, Acrosomal Integrity and Mitochondrial Activity of Cooled Stallion Semen

  • Dhafer M. Aziz
  • Heinrich Enbergs

Abstract

Fresh semen samples were collected from 11 warm blood stallions, each ejaculate was distributed into three equal parts. The first part was diluted in a skim milk-glucose diluent (SMG), the second part was diluted in a skim milk-glucose supplemented with Tyrode's medium (SMG-T), the third part was centrifuged to remove the seminal plasma, then the sperm was resuspended in the second diluent (SMG-T-C). The diluted semen were evaluated immediately after dilution (0 hour) and at 24, 48, 72, and 96 hours of storage at 5°C. Flow cytometry was performed to determine sperm viability, mitochondrial activity and acrosomal integrity. Immediately after dilution the tested parameters of sperms that diluted in SMG-T was significantly (P<0.001) higher than those diluted with SMG and SMG-T-C, and with SMG-T-C were higher significantly (P<0.05) than those diluted with SMG. The decreasing rate in tested sperm parameter was greater significantly (P<0.001) in semen samples which were diluted with SMG than those diluted with SMG-T and SMG-T-C. In conclusion, the present study indicated that viability, acrosomal integrity, and mitochondrial activity of stallion sperms were better preserved in SMG-T in comparison with SMG, also centrifugation and removal of the seminal plasma have an adverse effect on these three sperm parameters.

Published
2012-07-01
How to Cite
AZIZ, Dhafer M.; ENBERGS, Heinrich. Effect of Seminal Plasma Removal on Cell Membrane, Acrosomal Integrity and Mitochondrial Activity of Cooled Stallion Semen. Journal of Advanced Veterinary Research, [S.l.], v. 2, n. 3, p. 148-152, july 2012. ISSN 2090-6277. Available at: <https://advetresearch.com/index.php/AVR/article/view/171>. Date accessed: 16 dec. 2018.
Section
Original Research