Ziziphus spina-christi extract alleviate Cisplatin induced hepatorenal toxicity in rat via oxidative stress and apoptosis modulation

Abstract

Cisplatin is famous, effective anti-cancer drug but has some adverse effects such as nephrotoxicity and hepatotoxicity. This work was planned to assess the protective effect of Ziziphus spina-christi oil extract against Cisplatin induced hepatorenal toxicity.  A total of 24 male albino rats divided into equal four groups for 14 consecutive days. G1(control), G2(ziziphus spina-christi), G3(cisplatin), G4(cisplatin+ ziziphus spina-christi). Cisplatin treated rats showed increase serum of AST, ALP, ALT, triglyceride, cholesterol, LDL- cholesterol, urea, creatinine concentrations and decreased total protein and albumin concentration. Furthermore, cisplatin treated rats significantly increased the MDA level and decreased both of CAT and SOD levels in hepatic and renal tissue compared to control.  Administration of Ziziphus spina-christi oil extract orally at dose of with once intraperitoneal IP at 10^th day of experiment renormalized cisplatin hepatorenal damaged group by exerting antioxidant, anti-inflammatory and anticancer effects. Both of histopathological and immunohistochemical (TNF alpha) examination showed good changes, recorrection after using of Ziziphus Spina-Christi oil extract orally.