Avipox Virus Infection in Rosella parakeet (Platycercus sp.)
Three parakeets were brought for postmortem examination with a history of sudden death. The gross examination of birds revealed multiple light whitish nodules around the left eye, on the skin at the level of hock joint, on the anterior part of tracheal mucosa, congested lung and pallor liver. Impression smears from nodules revealed numerous heterophils, red blood cells, necrotic epithelial cells and bacterial colonies. Histopathological examination of nodules revealed eosinophilic intracytoplasmic inclusion bodies in the spinosal cells, epidermal hyperplasia, ballooning degeneration of spinosal cells and bacterial colonies. The virus was isolated and infection was produced on both chorioallantoic membrane and chicken embryo fibroblasts. Polymerase chain reaction was carried out and primer set designed from the 4b core protein gene of fowl pox virus revealed amplification at 578 bp. Suitable remedial measures were recommended against avipox virus infection and secondary bacterial infection.
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