Immunohistochemical Localization of Progesterone Receptors in the Non-pregnant One-humped Camel Uterus

Abstract

The current study aimed to localize immunohistochemically the cellular progesterone receptors in the non-pregnant one-humped camel uterus. Uterine tissue specimens from 10 she-camels during estrous were collected from local slaughterhouses and prepared for indirect immunoperoxidase staining using primary antibody for human progesterone receptor PR (clone 10A9). Nuclear signals for progesterone receptors were clearly observed in the uterine surface epithelium, glandular epithelium, myometrium, uterine stroma and in walls of some large blood vessels. The staining intensity was variable at different uterine tissue regions. Strong signals were demonstrated in the superficial gland zone, in the connective tissue stroma and in the myometrium. However, Nuclear signals of moderate intensity were noticed in the surface epithelium, deep gland zones and in the walls of the uterine arteries. No cytoplasmic signals could be detected. The current study concluded that nuclear signals for progesterone receptors were found in uterine surface epithelium, uterine glands, myometrium, uterine blood vessels and stroma.