Molecular detection of some antibiotic resistance genes of Escherichia coli isolated from bovines subclinical mastitis

Abstract

Antimicrobial drug resistance is considered an urgent major global public health threat facing humanity. With the rise in the prevalence and severity of both fatal and crippling illnesses, this crisis will have a catastrophic effect on human society. It doesn’t only affect public health but also causes serious problems in the dairy industry. The objectives of this study were to determine the prevalence, antibiotic resistance, and detection of Escherichia coli that produces extended-spectrum β-lactamase (ESBL) isolated from bovine subclinical mastitis cases. Escherichia coli was detected in 26 out of the 100 subclinical mastitis cases. The antibiotic sensitivity revealed that 10 from 26 isolated Escherichia coli were multidrug resistant. The isolates were most frequently resistant to amoxicillin (AMX) at 53.85 %, ampicillin (AMP) at 46.1%, cefotaxime (CTX) at 42.3% followed by amoxicillin-clavulanic acid (AMC) at 38.5 %. All the 26 Escherichia coli isolates were tested for Extended Spectrum b-lactamase by using the disc diffusion method, and the same 10 multidrug- resistant isolates were positive for Extended Spectrum b-Lactamases. All ten multidrug resistance and Extended Spectrum b-Lactamases Escherichia coli isolates were identified genetically by the PhoA gene and were found harboured b-Lactamases antibiotic resistance genes blaTEM 100%, blaCTXM 90%, blaSHV 80%, and ampC 80% respectively. The obtained results showed that phenotypic detection of 10 multidrug resistance and Extended Spectrum b-Lactamases isolates were agreed with genotypic molecular detection of b-Lactamases antibiotic resistance genes.