Making the most out of energy content in broiler diets using xylanase, emulsifier, and guanidinoacetic acid mixtures

Abstract

This research endeavour examined several nutritional approaches aimed at optimising the energy content of the broiler diets using mixtures with distinct mechanisms of action in low metabolisable energy diet (LME). Within this context, the impact of various xylanase (Xyl), emulsifier (EM), and guanidinoacetic acid (GAA) mixtures supplemented to LME diets were investigated on the following parameters: growth performance, energy and protein efficiency ratios, oxidative biomarkers, gene expression, and intestinal morphology. Seven hundred one-day-old (Ross 308) male-broilers were assigned to five experimental treatments (28 birds/replicate). The positive control group (PC) fed as breed-recommendations. A dietary change was made to the PC, where the dietary energy content was reduced by 200 kcal/kg feed, served as negative control group (NC). The other experimental diets were as in NC group, fortified with either Xyl+EM, Xyl+GAA or Xyl+EM+GAA. The results demonstrated a negative impact on the NC group’s productivity and biometric parameters compared to the PC group (p < 0.05). Combinations of Xyl+GAA or Xyl+EM+GAA were as effective as PC in term of growth performance (p > 0.05), but they were more efficient in terms of energy efficiency ratio (p < 0.05). In contrast to the PC group, The Xyl+EM group had lower protein efficiency ratio (p <0.05). The oxidative biomarkers, gene expression, and intestinal morphology of the NC groups supplemented with various mixtures were better than those of the PC group (p < 0.05). In conclusion, the overall benefits in the Xyl+EM+GAA group were notable. It is possible to compensate for LME (-200 kcal/kg feed) by using Xyl+EM+GAA mixture.