Impact of pH and temperature on bacteriocin activity and plantaricin C gene expression of Lactobacillus plantarum bacteria

Authors

  • Eman Gad Molecular Biology Researches & Studies Institute, Assiut University, Egypt
  • Walaa Elsherif Department of Food Hygiene and Nano-technological Research and Synthesis Unit at Animal health research institute, Agricultural Research Center, Assiut City, Egypt
  • Rania Ewida Department of Food Hygiene, Faculty of Veterinary Medicine, New Valley University, Egypt.
  • Bahaa Abd El-Fatah Department of Genetics, Faculty of Agriculture, Assiut University, Egypt.

Keywords:

Lactobacillus plantarum, bacteriocin, Plantacirin C, Real Time PCR, Milk product

Abstract

This study aimed to investigate the effect of different pH (7 and 4) and temperatures (4 and 40°C) on bacteriocin activity and plantaricin C (plnC) gene expression of Lactobacillus plantarium. Six strains of L. plantarum were used in these trials. The bacteriocin activity was measured after the different treatments by well diffusion test using indicator bacteria (Staphylococcus aureus and Escherichia coli O157:H7). Moreover, the plnC gene expression was determined by real-time PCR using 16S rRNA primers for universal bacteria and plantacirin C primers. The results declared a significant difference between the different pH and temperatures. In addition, the downregulation of plnC gene expression in pH 4. The upregulation of the same gene was applied to 40 ºC during bacteria incubation. Also, there is no correlation between the bacteriocin activity and plnC gene expression after applying different pH and temperatures in L. plantarum bacteria.

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Published

2024-10-02

How to Cite

Gad, E., Elsherif, W. ., Ewida, R., & Abd El-Fatah , B. . (2024). Impact of pH and temperature on bacteriocin activity and plantaricin C gene expression of Lactobacillus plantarum bacteria. Journal of Advanced Veterinary Research, 14(7), 1093-1095. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1928

Issue

Vol. 14 No. 7 (2024): October

Section

Original Research

License

Copyright (c) 2024 Journal of Advanced Veterinary Research

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