CMC-ase and β-Glucosidase activity of buffalo rumen cellulolytic bacteria isolates cultivated on a rice straw substrate at different incubation periods

Abstract

This study aimed to evaluate the activities of CMC-ase and β-glucosidase in buffalo rumen bacterial isolates cultured on a rice straw substrate at different incubation periods. The experiment followed a Completely Randomized Design with a 2 × 5 factorial pattern. The first factor was the bacterial isolate type (ST6 and ST8), while the second factor was the incubation period with five levels: T0 (0 days), T1 (3 days), T2 (6 days), T3 (9 days), and T4 (12 days). Each treatment was replicated four times. The observed variables were CMC-ase and β-glucosidase activities. Data were analyzed using one-way ANOVA, followed by Duncan's Multiple Range Test (DMRT). The results showed that the interaction between isolate type and incubation period had no significant effect on CMC-ase activity (P > 0.05). Neither factor independently influenced CMC-ase activity (P > 0.05), with an average enzyme activity of 0.78122±0.238 U/mL. Similarly, the interaction effect on β-glucosidase activity was not significant (P > 0.05). The type of isolate did not affect beta glucosidase activity, but differences in incubation time affected beta glucosidase activity with the best results at 6 days of incubation (0.030221 ± 0.008 U/mL).  In conclusion, the CMC-ase activity of buffalo rumen cellulolytic bacterial isolates cultured on a rice straw substrate is not affected by isolate type or incubation period, whereas β-glucosidase activity is influenced by incubation time.