Cryopreservation of Donggala bull semen in Andromed and CEP-2 diluents with the addition of egg yolk in different levels

Abstract

This research aimed to know at the quality of Donggala bull semen after thawing, as an effort to preserve local genetic resources in central Sulawesi. This research used five Donggala bulls who had been selected according to their performances. This research employed two kinds of basal diluents Andromed an CEP-2), with five different treatments for each. The diluent formula treatments were as follows: A1 = Andromed without egg yolk, A2 = Andromed 95% + egg yolk 5%, A3 = Andromed 90% + egg yolk 10%, A4 = Andromed 85% + egg yolk 15%, A5 = Andromed 80% + egg yolk 20%, C1 = CEP-2 without egg yolk; C2 = CEP-2 95% + egg yolk 5%; C3 = CEP-2 90% + egg yolk 10% and C4 = CEP-2 85% + egg yolk 15% and C5 = CEP-2 80% + egg yolk 20%. The variables observed were motility, viability and abnormality of spermatozoa after thawing. Based on the research results, it showed that the quality of fresh semen from Donggala bull has a volume (6 - !0 ml ± 1.2), colour (milky white to yellowish white), concentration (927x 106 to 1410 x 106), with motility (70 to 85 %). Meanwhile, the quality of semen after thawing with the best motility was an average of 58.68 ± 4.46 using Andromed diluent + 15% egg yolk. For C3 diluent, the highest motility (44.2%) was in CEP + egg yolk 10% diluent. The conclusion of this research is that Andromed diluent could maintain the quality of Donggala bull semen after thawing.