The PCR Identification of Trypanosomes Isolated from Cattle and Glossina spp. in Wildlife-Human-animal Interface of Meatu District, North-Eastern Tanzania

  • Deusdedit Malulu Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania
  • Huruma N Tuntufye Department of Microbiology, Parasitology and Biotechnology, Sokoine University of Agriculture, Tanzania
  • Benigni Temba Department of Veterinary Physiology, Biochemistry and Toxicology, Sokoine University, Tanzania
  • Elikira Kimbita Department of Microbiology, Parasitology and Biotechnology, Sokoine University of Agriculture, Tanzania
  • Imna Malele Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania
  • Safari Kinung'hi National Institute for Medical Research, Mwanza Centre, Tanzania
  • Hamisi Nyingilili Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania
  • Togolai Mbilu National Institute for Medical Research, Tabora Centre, Tanzania
  • Josephat S Kaboya National Institute for Medical Research, Tabora Centre, Tanzania
  • Eugene Lyaruu Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania

Abstract

African trypanosomes are etiological agents of trypanosomosis transmitted by tsetse flies (Glossina spp). Thus, identifying them in vectors and hosts together with their classification into species, subspecies is crucial for effective control of the diseases they cause to animals and human. This study analysed 350 samples collected from cattle (100) and tsetse flies (250) of Meatu district for identification of trypanosomes through amplification of Internal transcribed spacer 1 (ITS-1) region in order to support formulation of tsetse and trypanosomosis control strategies within the district. Occurrence of trypanosomes in cattle was 15%, while in tsetse was 1.20%. Trypanosoma congolense was identified in cattle whereas; T. simiae and T. godfreyi were identified in tsetse flies. The tsetse and trypanosomosis control options were discussed.

Author Biographies

Huruma N Tuntufye, Department of Microbiology, Parasitology and Biotechnology, Sokoine University of Agriculture, Tanzania

Lecturer (Deceased)

Benigni Temba, Department of Veterinary Physiology, Biochemistry and Toxicology, Sokoine University, Tanzania

Lecturer

Elikira Kimbita, Department of Microbiology, Parasitology and Biotechnology, Sokoine University of Agriculture, Tanzania

Professor 

Imna Malele, Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania

Institute Manager

Safari Kinung'hi, National Institute for Medical Research, Mwanza Centre, Tanzania

Institute Director

Hamisi Nyingilili, Vector and Vector-Borne Diseases Institute, Tanga,  Tanzania

Molecular Laboratory Manager

Josephat S Kaboya, National Institute for Medical Research, Tabora Centre, Tanzania

Laboratory technologist (Deceased)

Published
2019-04-06
How to Cite
MALULU, Deusdedit et al. The PCR Identification of Trypanosomes Isolated from Cattle and Glossina spp. in Wildlife-Human-animal Interface of Meatu District, North-Eastern Tanzania. Journal of Advanced Veterinary Research, [S.l.], v. 9, n. 2, p. 64-68, apr. 2019. ISSN 2090-6277. Available at: <https://advetresearch.com/index.php/AVR/article/view/355>. Date accessed: 24 apr. 2019.
Section
Original Research