Differential Leucocyte Count and Total Colony Count Changes in Heat Stressed Broiler

Abstract

The present study aimed to explain the role of additives in alleviation of the negative effect of heat stress on differential leucocytes count, heterophil / lymphocytic ratio with especial studies on the microbial count of duodenum, jejunum, ileum and caecum. A total of 270 day-old (Ross308) broiler chickens randomly divided into 6 groups, which were kept under elevated temperature (34-36â—¦C) and feed diet containing 1% Nigella Sativa (G2) or a mixture of sodium bicarbonate and potassium chloride (0.3% KCL+ 0.5% NaHCO3) (G3) or 2% coriander seed (G4) or 0.03% Acetylsalicylic acid (aspirin) (G5) or 250 mg of Ascorbic acid (Vitamin C) (G6) for 6 weeks. The previous parameters were recorded after slaughtering to take microbial sample and collecting blood parameters. The results explained that, there was a significant increase lymphocyte percentage in case of G2, G3, G4, G5 and G6. while, there was significant decreases in Heterophil percentage, heterophil/lymphocyte ratio (H/L), eosinophils percentage, monocytes percentage, jejunum total colony count and caecum total colony in case of (G2, G3, G4, G5), (G6, G2, G3, G4, G5), (G6, G3), (G5; G2), (G3; G2) and (G2, G4) respectively in compared with control group (P<0.05). It could be concluded that black seed and coriander seed have a positive effect on heat stress broiler diet.