The Association between POU1F1 Gene Polymorphisms and Growth as well as Carcass Traits of Noi Native Chickens
The study was conducted to detect and analyze the association between single nucleotide polymorphisms (SNP) in the POU1F1 (POU class 1 homeobox 1) gene and growth as well as carcass traits in Noi native chickens. Blood samples were taken at the wings, DNAs were extracted based on the phenol: chloroform technique and genotypes were analyzed by PCR-RFLP method. The frequencies of CC genotypes for three polymorphic sites (POU1F1_HhaI SNP, POU1F1_EcoRI SNP, POU1F1_BspHI SNP) were the highest. The corresponding C allele frequencies were higher than those of T alleles. Of three polymorphisms, POU1F1_BspHI SNP was found to be significantly linked with growth and carcass traits. Chickens bearing TT genotype showed higher body weight at 91 days, carcass weight, breast weight, and thigh weight than those of chickens with CC genotype. This SNP can be a useful marker for the selection of Noi chicken for improvement of growth and carcass traits.
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