Hydrogen Sulfide Ameliorative Role in Induced Diabetes in Rat by Regulating Endoplasmic Reticulum Stress Signaling and miRNA-27a

Authors

  • Hytham Saleh Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt, 41522.
  • Jihan A. Hasanen Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt, 41522.
  • Hanaa K. Ashour Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt, 41522.
  • Reham H. Alattar Fellow in the Health Affair, Student’s Hospital, Zagazig University, Zagazig, Egypt, 44511.
  • Tarek Khamis Pharmacology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt, 44511.
  • Khalifa El-Dawy Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt, 44511.

Keywords:

Type 2 diabetes, Hydrogen sulphide, endoplasmic reticulum stress pathway, insulin release, miRNA

Abstract

The present study was carried out to investigate the effects of garlic (Allium sativum Linn) and leek (Allium porrum L.) on biochemical parameters, lipid profile and gene expression in high fructose diet (HDF)- induced diabetes in rat. In this study, we used 80 males Wistar rats for 18 weeks, HDF was administered daily in diet to induce diabetes. A high and low dose of garlic oil and leek powder were given orally daily to HDF-diabetic rats. Compared to rats in the diabetic groups, the garlic oil and leek powder reduced serum cholesterol, triacylglycerol, low-density lipoprotein-cholesterol (LDL-c) and very low density lipoprotein-cholesterol (VLDL-c) levels. The garlic oil and leek powder also helped reduce hepatic destruction. A reduction was found in the gene expression in the hepatic homogenate of activating transcription factor 6 (ATF6) and X-Box Binding Protein1 (XBP1), Binding immunoglobulin protein (BIP), C/EBP homologous protein (CHOP), Jun N-terminal kinase (JNK), and peroxisome proliferator- activated receptor gamma (PPAR-γ). On the other hand, there was a significant upregulation in the mRNA expression of has been found in the promoter of glucose transporter 2 (Glut2), and miRNA 27a which is also a dose- and time-dependent manner. These results suggest that H2S donor as garlic oil and leek powder exhibits therapeutic potential for diabetes, which is most likely related to its protective effects against ER stress and regulating miRNA 27a and its target gene.

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Published

2023-08-20

How to Cite

Saleh, H. ., Hasanen, J. A. ., Ashour, H. K. ., Alattar, R. H. ., Khamis, T. ., & El-Dawy, K. . (2023). Hydrogen Sulfide Ameliorative Role in Induced Diabetes in Rat by Regulating Endoplasmic Reticulum Stress Signaling and miRNA-27a. Journal of Advanced Veterinary Research, 13(7), 1351-1358. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1389

Issue

Vol. 13 No. 7 (2023): Special Issue: The 6th Scientific Conference on Food Safety (Human and Food Hygiene), Food Safety, Hygiene & Technology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt (3/5/2023).

Section

Original Research

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Copyright (c) 2023 Journal of Advanced Veterinary Research

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