Promoting Efficacy of Human Platelet Lysate on Proliferation of Mammalian Cell Lines Used for Vaccine Production

Authors

  • Naglaa. I. Aly Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt.
  • Hossam. M. Fahmy Laboratory and Transfusion Medicine, Ain Shams Medical School, Cairo, Egypt.
  • Omima Atta Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt.
  • Mohamed Elzallat Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt.
  • Amany. M. Abass Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt.
  • Hoda Abu-Taleb Environmental Research Department, Theodor Bilharz Research Institute, Giza, Egypt.

Keywords:

Human Platelet Lysate (HPL) , Growth factor , Cell culture

Abstract

The current study was conducted to determine to what extent human platelet lysate (HPL) can be used to enhance the growth of three cell lines used for propagation of Bovine Ephemeral Fever Virus (BEFV) and Rabies virus (RV) for vaccine production. HPL; Fetal Calf Serum (FCS) and Newborn Calf Serum (NBCS) were used as supplements to the used cell culture medium (Minimum Essential Medium “MEMâ€) for passaging of three cell lines including baby hamster kidney (BHK-21); African green monkey kidney (VERO) and Madin Derby canine kidney (MDCK). Through three successive cell culture passages; for each cell line; it was found that HPL supported growth and propagation of BHK-21, VERO and  MDCK cell lines without altering their morphologic and functional characteristics. It has been accepted as a suitable alternative to FBS and NBCS, and it can be used in vaccinal production.

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Published

2023-08-08

How to Cite

Aly, N. I. ., Fahmy, H. M. ., Atta, O. ., Elzallat, M. ., Abass, A. M. ., & Abu-Taleb, H. . (2023). Promoting Efficacy of Human Platelet Lysate on Proliferation of Mammalian Cell Lines Used for Vaccine Production. Journal of Advanced Veterinary Research, 13(6), 1157-1160. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1398

Issue

Vol. 13 No. 6 (2023): Special Issue: The 6th Scientific Conference on Food Safety (Human and Food Hygiene), Food Safety, Hygiene & Technology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt (3/5/2023).

Section

Original Research

License

Copyright (c) 2023 Journal of Advanced Veterinary Research

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