Contamination of chicken meat and edible offal commercialized at retail in Sharkia Governorate, Egypt by Enterobacteriaceae

Abstract

Chicken meat and its edible offal have a high biological value and act as a good substrate for different types of bacteria implicated in foodborne disease outbreaks. Therefore, a total of 150 random samples of chicken (Breast, thigh and edible offal, 50 of each) were collected from different outlets, Sharkia Governorate, Egypt to be examined bacteriologically. The obtained results revealed that the mean Enterobacteriacae count was 3.73±0.07, 4.02±0.10 and 4.34±0.12 log₁₀ CFU/g in breast, thigh and edible offal samples, respectively. E. coli was isolated from 12(24%), 15(30%) and 20(40%) of breast, thigh and edible offal samples, respectively, five different serotypes were identified (O157:H7, O158:H19, O128:H2, O26:H11and O55:H7) and the isolated E. coli strains were resistant to penicillin (100%), while the resistance was 72.3%, 65.9%, 51.1% and 51.1% to sulphamethoxazol-trimethoprim, oxytetracyclin, chloramphenicol and kanamycin, respectively, meanwhile, all strains were sensitive to amoxicillin-clavulanic acid. Salmonella spp. were isolated from 22(14.67%) of the examined samples with a prevalence of 5(10%), 7(14%) and 10(20%) in breast, thigh and edible offal, respectively, serological identification revealed five different serotypes (S. typhimurium, S. entritides, S. lindenberg, S. infantis and S. kentukay), and the isolated Salmonella spp. were resistant to penicillin and sulphamethoxazol-trimethoprim (100%), meanwhile, the sensitivity was 100% to amoxicillin-clavulanic acid and ampicillin.