The effect of encapsulated Japanese Papaya leaf extract supplementation in diets on protein digestibility, immunity, and performance of broiler chickens

Abstract

The research propose to evaluate the impact of addition encapsulated Japanese papaya leaf extract (EEDPJ) to diets on protein digestibility, health status, and broiler performance. This study used 200 unsexed day-old Ross 308 broiler chickens with fully randomized design, four treatments, five repetitions, and each comprising 10 birds. The treatments were as follows: T0, basal diet (control); T1, basal diet + 0.09% Japanese papaya leaf extract (EDPJ); T2, basal diet + 0.03% EEDPJ; T3, basal diet + 0.06% EEDPJ and T4, basal diet + 0.09% EEDPJ. Measurement the parameters using total lactic acid bacteria (LAB), total coliforms, small intestine pH, protein digestibility, lymphoid organ weight, heterophil/lymphocyte ratio (H/L), feed intake, daily weight gain, and feed conversion ratio (FCR). Data analysis used variance analysis and Duncan's Multiple Range Test at 5% significance. Supplementation with EEDPJ gives effect to led a significant improvement (P<0.05) in daily weight gain, feed conversion ratio (FCR), lactic acid bacteria population, coliform count, intestinal pH, relative weights of the bursa of Fabricius and spleen, heterophil/lymphocyte (H/L) ratio, and protein digestibility. In contrast, not significantly influenced (P>0.05) on daily feed intake and thymus weight. In inclusion, the addition of 0.06% EEDPJ in the diet enhanced protein digestibility, strengthened immune response, and improved the broiler chickens growth performance.