Fermentability and total digestible nutrients of Pennisetum purpureum cv mott supplemented with buffalo rumen content as probiotic and various level of readily available carbohydrate in balibul sheep in vitro

Abstract

The purpose of the study was to produce probiotic origin from buffalo rumen-contents enriched with N, S, P, and cassava flour as a source of readily available carbohydrate (RAC) to increase fermentability and energy production of Pennisetum purpureum cv Mott grass in the rumen of balibul sheep. Materials used in this study were Pennisetum purpureum cv Mott grass, buffalo rumen-contents, elements N, S and P and cassava flour. The study was conducted in vitro using a completely randomized design (CRD) consisting of 4 treatments and 4 replications. Variables measured were total volatile fatty acids (VFA), ammonia (NH3) and total digestible nutrients (TDN). Results of the study showed that supplementation of cassava flour as a source of RAC significantly increased the production of VFA, and TDN value (P<0.05), but significantly decreased NH3 production. Average VFA production were 56.67 (T0), 63.33 (T1), 73.33 (T2) and 66.67 mM. Average NH3 production were 5.61 (T0), 5.28 (T1), 5.06 (T2) and 5.04 mM. Average TDN value were were 50.47 (T0), 5.28 (T1), 5.06 (T2) and 5.04%. It can be concluded that supplementation of cassava flour as a source of RAC increase VFA production and TDN value whith supplementation of 5% RAC and reduces NH3 production.