Molecular Characterization of Biofilm Producing Genes in Salmonellae Isolated from Chicken

  • Marwa Ragab Abd El-basit Reference Laboratory for Veterinary Quality Control on Poultry production (RLQP), Animal Health Research Institute, Luxor, Egypt.
  • Mohamed Wael Abd El-Azeem Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.
  • Serageldeen Sultan Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.
  • Soad A. Nasef Reference Laboratory for Veterinary Quality Control on Poultry production (RLQP), Animal Health Research Institute, Dokki, Giza 12618, Egypt.

Abstract

Salmonella enterica considered one of the most important food-borne pathogen. Biofilm formation considered one of the main problems related to S. enterica, in this study, biofilm formation, colony morphotype, cellulose and curli production genes of 19 Salmonella isolates were tested. The results showed that 85% of isolates produced strong biofilm and 15% of isolates produced moderate biofilm on polystyrene plate with 1/20 diluted TSB. Different colony morphotypes expressed saw, sbam, and rdar morphotype when cultivated on LB containing Congo red for monitoring cellulose and curli production. All S. enterica strains possess adrA, csgD and gcpA genes using PCR. Thus in this study all Salmonella isolates formed biofilm so they give increased tolerance for antimicrobial agents and disinfectant, which results in difficulty in the treatment of diseases and causing many problems in food industry as it becomes a persistent of source of contamination.

Published
2019-03-21
How to Cite
ABD EL-BASIT, Marwa Ragab et al. Molecular Characterization of Biofilm Producing Genes in Salmonellae Isolated from Chicken. Journal of Advanced Veterinary Research, [S.l.], v. 9, n. 2, p. 39-44, mar. 2019. ISSN 2090-6277. Available at: <https://advetresearch.com/index.php/AVR/article/view/341>. Date accessed: 26 aug. 2019.
Section
Original Research