Investigation of Antibacterial Efficiency of Various Lytic Bacteriophages Isolated from Chickens Against Characterized Multidrug-resistant Pathogenic Bacterial Strains

Authors

  • Marwa Fathy Department of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
  • Afaf Ahmed Reference Laboratory for Quality Control on Poultry Production (RLQP), Agriculture Research Center (ARC) / Animal Health Research Institute (AHRI), Luxor, Egypt.
  • Mohamed Wael Abd El-Azeem Department of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
  • Sabry Hassan Department of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
  • Serageldeen Sultan Department of Microbiology, Virology Division, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.

Keywords:

Bacteriophages, Multidrug-resistant bacteria, Chicken, EOP, TEM, PCR

Abstract

This study targeted isolation and characterization of potential bacteriophages (phages) infecting MDR pathogenic bacteria recovered from chickens and analyzed their efficacy as bio-control agents. A total of 45 different bacterial isolates (18 E. coli, 16 Salmonellae spp., 5 Staphylococcus spp., 2 Pseudomonas spp., 1 Proteus mirbalis, 1 Citrobacter spp., 1 Enterobacter aerogenes and 1 Klebsiella pneumonia) were obtained from chickens in the current study and previous studies. The identified isolates were investigated for the presence of virulence genes and MDR using PCR and disc diffusion method, respectively. Nine purified phages classified morphologically into 3 families (Myoviridae, Siphoviridae and Podoviridae) using Transmission Electron Microscope were recovered from chicken intestinal contents and showed viability at wide pH range, resistance to organic solvents and thermostability at high temperatures (up to 80ºC). The potential phages exhibited various bacterial host ranges using the spot test and the efficiency of plating (EOP) assay. The results revealed the prevalent of pathogenic E. coli and salmonella serovars among the recovered isolates with different virulence and genotypic patterns. The lytic phages were highly stable and have the capacity to infect different pathogenic MDR bacterial strains. This study demonstrated that these promising phages of avian origin could be used to control the pathogenic MDR E. coli and Salmonella serovars which possess public concerns on human health and poultry industry.

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Published

2022-06-27

How to Cite

Fathy, M., Ahmed, A., Wael Abd El-Azeem, M., Hassan, S., & Sultan, S. (2022). Investigation of Antibacterial Efficiency of Various Lytic Bacteriophages Isolated from Chickens Against Characterized Multidrug-resistant Pathogenic Bacterial Strains. Journal of Advanced Veterinary Research, 12(3), 265-277. Retrieved from https://advetresearch.com/index.php/AVR/article/view/979

Issue

Section

Original Research

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