Silver Nanoparticles and Sodium Hypochlorite Inhibitory Effects on Biofilm Produced by Pseudomonas aeruginosa from Poultry Farms

Abstract

In Egypt, pseudomonas infection is one of the most important problems facing poultry production. Pseudomonas spp. is recognized as a major food spoiler and environmental contaminant. Biofilm formation by P. aeruginosa has an important role in the bacterial pathogenesis as well as persistence in the environment. The antibacterial and antibiofilm activities of AgNPs and NaOCL were evaluated against P. aeruginosa isolated from chicken farms. MIC and MBC of AgNPs against planktonic cells of P. aeruginosa were 15 and 20µg/ml, respectively. While those of NaOCL were 2200 and 2600 µg/ml, respectively. The highest inhibition percentages of biofilm formation (97.9%) was observed when P. aeruginosa treated with AgNPs (25µg/ml). While, 87.5% biofilm removal percentage was achieved after treating the established biofilm with 25 µg/ml AgNPs for 2.5 h. Moreover, NaOCL (2800 µg/ml) was able to cause 96.6% inhibition of biofilm formation and 90.3% biofilm removal after 1.5 h contact. The current study revealed that AgNPs and NaOCL were able to promote a significant reduction and removal of the mature biofilms formed by P. aeruginosa and the antibiofilm efficiency increased with the increase of its contact times with the biofilms.