Assessment of Microbial Safety and Quality of Market Raw Milk and Pasteurized Milk Sold in Dakahlia Governorate, Egypt

Authors

  • Shaimaa Abdelnasser Ahmed Animal Health Research Institute , Mansoura Provincial Laboratory, Agricultural Research Center , Ministry of Agriculture, Egypt.
  • Adel Hassanin Mahmoud Mostafa Animal Health Research Institute , Mansoura Provincial Laboratory, Agricultural Research Center , Ministry of Agriculture, Egypt.
  • Mohammed El-Sherbini Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Adel Abdelkhalek Food Safety, Hygiene and Technology Department, School of Veterinary Medicine, Badr University, Cairo, Egypt.

Abstract

In this study, assessing the microbial quality and safety of the market raw milk and pasteurized milk is the basal objective of this study. One hundred and twenty (120) milk samples (60 of each) were collected randomly from different supermarkets and retailer shops in Dakahlia governorate, Egypt. Samples were analyzed for Total bacterial count (TBC), total coliforms (TCC), Total Staphylococcus count (TSC), and Total yeast and mould count. All market milk samples, and pasteurized milk were found to be contaminated and judged poor. Listeria spp. were isolated on Oxford agar and then subjected to biochemical and molecular identification. The overall isolation rate of Listeria spp. from the market raw milk was 28.33%. The prevalence rates of Listeria monocytogenes were 13.33% in raw market milk. The prevalence of Listeria innocula and other Listeria species were 3.33% and 11.67%, respectively. Polymerase chain reaction (PCR) was used to detect the virulence genes (16SrRNA, hylA, and prfA) of 8 biochemically identified L. monocytogenes strains recovered from raw market milk using specific primers. 16SrRNA, hylA, and prfA genes are considered the best indicator for virulence determination of L. monocytogenes isolated from market raw milk. A high microbial load of market milk and pasteurized milk may present a public health hazard to the consumers and emphasizes the need for improved hygienic standards.

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Published

2022-09-17

How to Cite

Abdelnasser Ahmed, S. ., Hassanin Mahmoud Mostafa, A. ., El-Sherbini, M. ., & Abdelkhalek, A. . (2022). Assessment of Microbial Safety and Quality of Market Raw Milk and Pasteurized Milk Sold in Dakahlia Governorate, Egypt. Journal of Advanced Veterinary Research, 12(4), 456-461. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1068