Nephroprotective effect of Alogliptin and L-Carnitine in Gentamicin-induced Toxicity in male Albino rats

Abstract

           The purpose of this investigation is the supportive functions of L-carnitine when co-administered with the DDP-4 inhibitor member and the nephroprotective impact of alogliptin in nephrotoxicity induced by gentamicin in male Wistar rats. A  total number of 25 male Albino rats weighed 130 ± 5.7 g. and divided into five equal  groups, G1:(control negative), G2: (control positive, have nephrotoxicity), G3: (treated group with L-carnitine), G4: (treated with Alogliptin) and G5: (treated with alogliptin and L-carnitine). The biochemical results of serum total proteins, albumin, urea, uric acid and creatinine showed significant changes (P ≤ 0.05) among different groups while serum globulin is not significant and the mean value of these parameters showe the significant of G5 than G 3 and G4. The following measures were made in order to identify the oxidative/antioxidant cascades: glutathione peroxidase (GPx), catalase (CAT);, concentration of malondialdehyde (MDA) and Super Oxide Dismutase (SOD) and  these parameters were showed significant changes (P ≤ 0.05)   among different groups and the mean value showed the significant  of G5 than G 3 and G4. The results of the histological and immunohistochemical analyses point to a possible function for alogliptin and L-carnitine in preventing renal tissue destruction in rat models of gentamicin-induced nephrotoxicity. According to the improvement of various biochemical indicators and oxidative state as well as the restoration of the renal structural integrity and function, treatment with alogliptin or L-carnitine protects the kidney through their antioxidant action. Better results than each medicine alone are seen when the two are combined.