Supplementation of alpha lipoic acid on Kacang goat (Capra hircus) oocyte to the growth of GDF-9 and BMP-15

Abstract

The application of assisted reproductive technology by mass-producing embryos is to maintain genetic resistance, increase productivity and livestock populations in a short time with maximum quality. Oocyte In Vitro Maturation (IVM) is a crucial stage of In Vitro Fertilization (IVF) implementation to increase the quantity and quality of livestock genetic trait through in In Vitro Embryo Production (IVP). GDF-9 and BMP-15 expression are in the family of Transforming Growth Factor Beta (TGF-β) which are known to regulate oocyte and cumulus cell development in mammals. This study's objective was to investigate the effects of Alpha Lipoic Acid (ALA) on IVM media of Kacang goat (Capra hircus) oocytes. This research was an experimental study design consisting of 3 treatment groups: (P0) the control group, (P1) 25 µM/L ALA supplementation and (P2) 50 µM/L ALA supplementation. This study used 261 COCs which were 22 hours matured in vitro at 38.5°C, 98% humidity, and 5% CO2. GDF-9 and BMP-15 expression data were statistically tested with a significance of p<0.05. Immunocytochemistry showed the expression value of GDF-9 (2.86 ± 1.06a, 4.29 ± 1.79a and 8.29 ± 2.69b). BMP-15 expression values (2.57±1.39a, 5.71±3.72b and 11.43±1.51c). The results of the study showed that supplementation with ALA on maturation media at a dose of 50 µM/L toward the maturation level and increased GDF-9 and BMP-15 expression in vitro maturation of Kacang goat oocyte.