Supplementation of alpha lipoic acid on Kacang goat (Capra hircus) oocyte to the growth of GDF-9 and BMP-15

Authors

  • Laily Laily U.N. Ilmi Master Program of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-7269-0755
  • Tita Damayanti Lestari Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-8561-3452
  • Widjiati Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-8376-1176
  • Erma Safitri Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-6817-1178
  • Tjuk Imam Restiadi Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-9382-6546
  • Maslichah Mafruchati Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-6010-1984
  • Muhammad Fajar Amrullah Master Program of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0003-1589-4572
  • Aswin Rafif Khairullah Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-9421-9342
  • Shendy Canadya Kurniawan Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, Netherlands https://orcid.org/0000-0003-0470-3363
  • Salsabilla Abani Master Program of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0009-0003-7994-7036
  • Abdullah Hasib School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Queensland https://orcid.org/0000-0002-4188-0492
  • Muhammad Esa Erlang Samodra Bachelor Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0009-0000-0472-7248
  • Otto Sahat Martua Silaen Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia https://orcid.org/0000-0002-5170-6797

Keywords:

In vitro maturation, alpha lipoic acid, reproductive healthcare, GDF-9, BMP-15

Abstract

The application of assisted reproductive technology by mass-producing embryos is to maintain genetic resistance, increase productivity and livestock populations in a short time with maximum quality. Oocyte In Vitro Maturation (IVM) is a crucial stage of In Vitro Fertilization (IVF) implementation to increase the quantity and quality of livestock genetic trait through in In Vitro Embryo Production (IVP). GDF-9 and BMP-15 expression are in the family of Transforming Growth Factor Beta (TGF-β) which are known to regulate oocyte and cumulus cell development in mammals. This study's objective was to investigate the effects of Alpha Lipoic Acid (ALA) on IVM media of Kacang goat (Capra hircus) oocytes. This research was an experimental study design consisting of 3 treatment groups: (P0) the control group, (P1) 25 µM/L ALA supplementation and (P2) 50 µM/L ALA supplementation. This study used 261 COCs which were 22 hours matured in vitro at 38.5°C, 98% humidity, and 5% CO2. GDF-9 and BMP-15 expression data were statistically tested with a significance of p<0.05. Immunocytochemistry showed the expression value of GDF-9 (2.86 ± 1.06a, 4.29 ± 1.79a and 8.29 ± 2.69b). BMP-15 expression values (2.57±1.39a, 5.71±3.72b and 11.43±1.51c). The results of the study showed that supplementation with ALA on maturation media at a dose of 50 µM/L toward the maturation level and increased GDF-9 and BMP-15 expression in vitro maturation of Kacang goat oocyte. 

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Published

2023-12-31

How to Cite

Laily U.N. Ilmi, L., Damayanti Lestari, T., Widjiati, Safitri, E., Imam Restiadi, T., Mafruchati, M., Fajar Amrullah, M., Rafif Khairullah, A., Canadya Kurniawan, S., Abani, S., Hasib, A., Esa Erlang Samodra, M., & Sahat Martua Silaen, O. (2023). Supplementation of alpha lipoic acid on Kacang goat (Capra hircus) oocyte to the growth of GDF-9 and BMP-15. Journal of Advanced Veterinary Research, 14(1), 135-138. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1636

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Section

Original Research

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