Molecular detection of blaTEM gene for encoding extended spectrum beta-lactamase (ESBL) on Escherichia coli isolated from deer feces in Indonesia

Authors

  • Katty Hendriana Priscilia Riwu Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika. Jl. Pemuda No. 59A, Dasan Agung Baru, Mataram 83125, West Nusa Tenggara, Indonesia https://orcid.org/0000-0003-0395-2296
  • Mustofa Helmi Effendi Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-9727-411X
  • Fedik Abdul Rantam Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-1047-2410
  • Aswin Rafif Khairullah 4Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46 Cibinong, Bogor 16911, West Java, Indonesia. https://orcid.org/0000-0001-9421-9342
  • Shendy Canadya Kurniawan Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, Netherlands https://orcid.org/0000-0003-0470-3363
  • Ahmad Kurniawan College of Medical, Veterinary and Life Science, University of Glasgow, Glasgow G12 8QQ, United Kingdom https://orcid.org/0000-0001-8467-1159
  • Ikechukwu Benjamin Moses Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480211, Nigeria https://orcid.org/0000-0002-5928-8198
  • Abdullah Hasib School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Queensland https://orcid.org/0000-0002-4188-0492
  • Agus Widodo Department of Health, Faculty of Vocational Studies, Universitas Airlangga. Jl. Dharmawangsa Dalam Selatan No. 28-30, Kampus B Airlangga, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0003-3255-6188
  • Sheila Marty Yanestria Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya. Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Dukuh Pakis, Surabaya 60225, East Java, Indonesia https://orcid.org/0000-0002-2514-1104
  • Otto Sahat Martua Silaen Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia. Jl. Salemba Raya No. 6 Senen, Jakarta 10430, Indonesia https://orcid.org/0000-0002-5170-6797
  • Amir Shehzad Livestock and Dairy Development Department Government of Punjab, Anarkali Bazaar Lahore, Punjab 54000, Pakistan. https://orcid.org/0000-0002-8452-0294

Keywords:

E. coli, ESBL, deer, blaTEM, public health

Abstract

Antibiotic resistance is a serious global health threat and broad-spectrum beta-lactamase- producing Enterobactericeae (ESBL) are major contributors. This research focuses on the presence of Eschericia coli as a producer of ESBL in the fresh feces of deer. This study aims to gain deeper insight into ESBL sourced from deer. 129 fresh stool samples were collected. A total of 89 samples came from the East Java region and 40 samples came from the East Nusa Tenggara region. Identification were carried out which was then followed by PCR. We found 9 positive samples of ESBL-producing E. coli containing the blaTEM gene as the ESBL encoding gene. 33 samples (25.28%) were positive for E. coli. 8 isolates (24.24%) were multidrug-resistant, East Java (6 isolates) and East Nusa Tenggara (2 isolates). All isolates of multidrug-resistant E. coli 7 isolates were ESBL positive (87.5%) and also contained the blaTEM gene. The presence of MDR and ESBL-producing E. coli isolates have been confirmed in deer. Deer should be considered as a source of MDR and ESBL transmission for public health. The conclusion of this study is to reveal the presence of ESBL-producing E. coli originating from deer. The importance of this cross-sector collaboration to monitor antimicrobial resistance.

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Published

2024-04-07

How to Cite

Riwu, K. H. P., Effendi, M. H., Rantam, F. A., Khairullah, A. R., Kurniawan, S. C. ., Kurniawan, A., Moses, I. B. ., Hasib, A., Widodo, A., Yanestria, S. M. ., Silaen, O. S. M. ., & Shehzad, A. (2024). Molecular detection of blaTEM gene for encoding extended spectrum beta-lactamase (ESBL) on Escherichia coli isolated from deer feces in Indonesia. Journal of Advanced Veterinary Research, 14(4), 722-726. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1829

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Section

Original Research

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