Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia

Authors

  • Alfiana Laili Dwi Agustin Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-5467-6167
  • Fidi Nur Aini Eka Puji Dameanti Microbiology and Immunology Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Veteran, Ketawanggede, Lowokwaru, Malang 65145, East Java, Indonesia https://orcid.org/0000-0002-7928-1073
  • Mustofa Helmi Effendi Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-9727-411X
  • Wiwiek Tyasningsih Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0003-0604-8534
  • Aswin Rafif Khairullah Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0001-9421-9342
  • Shendy Canadya Kurniawan Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, Netherlands https://orcid.org/0000-0003-0470-3363
  • Ikechukwu Benjamin Moses Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480211, Nigeria https://orcid.org/0000-0002-5928-8198
  • Abdullah Hasib School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Queensland https://orcid.org/0000-0002-4188-0492
  • Yolla Rona Mustika Master Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0003-4832-3901
  • Kurnia Nisa Kinasih Master Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia https://orcid.org/0000-0002-3512-2222

Keywords:

Bats, multidrug resistance, E. coli, antibiotic, public health

Abstract

Wild animals such as bats usually do not receive antibiotic therapy, however bats can have bacteria that have developed resistance to antibiotics due to water contamination from pharmaceutical factory waste disposal, expired medicines that are thrown away carelessly, feces and urine that pollute the environment. This research aimed to look at cases of multidrug resistance (MDR) in bats on the island of Lombok, Indonesia. A total of 135 bat rectal swab samples were taken using sterile cotton buds. Isolation of E. coli bacteria using Eosin Methylene Blue Agar media then looking at morphology and biochemical tests. The bacterial sensitivity test used the disk diffusion method using seven antibiotics, namely the antibiotics amoxicillin, ciprofloxacine, trimethoprim/sulfamethoxazole, tetracycline, gentamicin, cefotaxime and azithromycin. The research results showed that from 135 samples tested, 97 samples were positive for E. coli. Samples that were positive for E. coli were tested for sensitivity, showing that 41 (41/97, 42.26%) samples were still sensitive to all tested antibiotics and 56 samples experienced resistance with the results of samples experiencing resistance to the antibiotic azithromycin (AZM) as many as 40 samples ( 41.2%), amoxicillin (AML) 25 samples (25.7%), tetracycline (TE) 23 samples (23.7%), sulfamethoxazole/trimethoprim (SXT) 21 samples (21.6%), ciprofloxacine (CIP) 14 samples (14.4%), gentamicin (CN) 1 sample (1%), and for cefotaxime (CTX) 0 samples experienced resistance (0%), and 15 samples experienced multidrug resistance. These results explain that bats in caves on the Indonesian island of Lombok act as transmitters of MDR E. coli to public health. 

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Published

2024-04-03

How to Cite

Agustin, A. L. D., Dameanti, F. N. A. E. P. ., Effendi, M. H., Tyasningsih, W. ., Khairullah, A. R., Kurniawan, S. C. ., Moses, I. B. ., Hasib, A., Mustika, Y. R., & Nisa Kinasih, K. (2024). Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia. Journal of Advanced Veterinary Research, 14(4), 683-686. Retrieved from https://advetresearch.com/index.php/AVR/article/view/1769

Issue

Vol. 14 No. 4 (2024): April

Section

Original Research

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