Duplex-PCR for Identification and Differentiation of Cattle and Buffalo Processed Meat

Authors

  • Rohita Gupta
  • D.N. Rank
  • C.G. Joshi

Keywords:

Meat speciation; Polymerase chain reaction; Cattle; Buffalo, cyt b

Abstract

A simple and reliable duplex polymerase chain reaction (duplex-PCR) technique is proposed to identify and differentiate cattle and water buffalo DNA using primers that were tested on mitochondrial DNA (mtDNA) extracted from meat muscle samples. Different levels of autolysis were experimentally produced by putrefaction and heating the samples at various temperatures and conditions to simulate the various meat processing technology. The optimized PCR amplified 113 bp and 152
bp fragment of cyt b gene from mtDNA. This test was successful in detecting up to 1 pg adulteration in cattle-buffalo meat mixture. The test is a valuable tool for meat authentication and screening of cooked, putrefied and mixed samples of cattle and buffalo.

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Published

2011-04-01

How to Cite

Gupta, R., Rank, D., & Joshi, C. (2011). Duplex-PCR for Identification and Differentiation of Cattle and Buffalo Processed Meat. Journal of Advanced Veterinary Research, 1(1), 13-16. Retrieved from https://advetresearch.com/index.php/AVR/article/view/208

Issue

Vol. 1 No. 1 (2011): April

Section

Original Research

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license