Immunophenotyping Panel of T Lymphocyte in Water Buffalo Spleen (Bubalus Bubalis) And Its Significant Correlation to Ruminant Diseases

Abstract

Spleen occupies an effective immunological role in mammals especially economic animals like water buffalo. Regardless of that, not literature points attention to immune cells homing buffalo spleen. This study sheds light on the distributional panel of T lymphocyte in water buffalo as a framework to easily diagnose any alteration in spleen composition that may refer to immunological disorders. Twenty spleens of both sexes, 3.0 ±0.5 years and 400.0 ±50.0 kgs were selected randomly from apparently healthy animals. Tissues were subjected to T cell antibodies via applying immunohistochemistry staining protocol. Quantitative analysis of the distribution of these antibodies was assessed by image J program. Area percentage of each antibody was evaluated by aid of R- program. CD3 was expressed for all T lymphocytes, while CD4, CD8, and CD45RO referred to a helper, cytotoxic, and memory T cells, respectively. Expression of CD3⁺, CD4⁺, and CD8⁺ cells was high in the periarterial lymphatic sheath, red pulp, and marginal zone with a significant area % difference than lymph nodule. Unlikely, CD45RO⁺ expression gained insignificant area %.  Most likely the obtained findings will give a reference scheme for T lymphocyte in buffalo spleen. This research presented a quantitative analysis to the distribution of distinct types of T lymphocyte in normal splenic tissue of water buffalo species.