Prominent Reaction to Tissue Factor Antibody in Hemal Node of Egyptian Water Buffalo (Bubalus bubalis)

Abstract

Tissue Factor (TF) histology is an essential prognosticator of hemostasis and outcomes in local thrombus. Fresh tissue testing for pathologist evaluation is the best loyal technique for histology categorization, in spite of this, no literature in histology allude to the characteristics and the distribution of TF in hemal node of water buffalo species. In this research paper, author proposes an original approach to predicting cellular allocation to TF antibody in hemal node. Over the branches of iliac arteries of the abdominal aorta and superficial temporal arteries of temporal region, samples were validated on a dataset comprising 7-12 fresh hemal nodes per healthy water buffalo receiving Islamic slaughtering. Samples were fixed in 10% neutral buffered formalin, sections were organized and stained for routine histological interpretations, and anti-TF antibody was utilized for immunofluorescence examination. Hemal node held a small peanut size. It was bordered with a connective tissue capsule fashioned up chiefly of collagen fibers along with few reticular fibers, and smooth muscle cells. Short trabeculae existed from capsule splitting the areas of subcapsular sinus. Inside hemal node parenchyma, lymphoid follicles and blood sinusoids were spontaneously scattered. Immunofluorescence staining highlighted the positive TF expressions to a population of cells homing the capsule, trabeculae, and lymphoid cords. Whereas a few positive TF expression remained demarking the bounds of lymphoid follicles. Hopefully, this research has the potential to enhance new approaches and provide a helpful reference for diagnosticians to monitor any morphological alterations in TF locality and elucidate the pathophysiology of life threating diseases in ruminant.