Utilization of hepatocyte cell culture to increase the success of in vitro fertilization in cattle

Abstract

This study aimed to determine levels of IGF-1 and estrogen obtained from hepatocyte cells culture for bovine in vitro fertilization. Hepatocyte cells were cultured in TCM 199 + FCS 10% + BSA 10% to produce IGF-1, estrogen and progesterone. Progesterone concentration, was minimized by binding technique using antiprogesterone coated polystyrene tubes. The IGF-1 and estrogen are used as supplementation media for in vitro fertilization and as embryo culture media. The result showed that 6 days incubation period had the most confluent monolayer compared to the other incubation period, therefore, resulted in the highest concentration of IGF-1 as well as estrogen. AntiP4 could be used to absorb progesterone produced from monolayer culture of liver and cumulus cells. IGF-1 and estrogen from monolayer culture of hepatocyte cells before and after progesterone absorption resulted in a cleavage rate of 27.48% and 53.61%, respectively. The stage morula embryo rates were 5.73% and 27.59%, and could also reduce the apoptosis in the embryos cultured in liver cell were 23.71%, while without supplemented apoptotic rate in embryo cell was 70.58%. It could be concluded that monolayer culture of bovine hepatocyte cells could produce IGF-1 and estrogen growth factor which acted as mitogenic substances that could be used as supplement media in in vitro fertilization and embryo development as well as able to reduce the incidence of apoptosis in embryo resulted.