Molecular characterization and identification of Infectious Laryngotracheitis Virus from clinical samples of poultry flocks in Indonesia

Abstract

Infectious Laryngotracheitis (ILT) in chickens is a significant issue that demands attention in Indonesia. This highly contagious respiratory disease caused by herpes requires urgent study and action. Despite the lack of confirmed ILT virus (ILTV) infection in layer hens in Indonesia, it is crucial to understand and address this potential threat. This study was dedicated to detecting and characterizing ILTV in layer hens from Bogor, Bekasi, Cianjur, and Tangerang districts for the first time in Indonesia, utilizing robust molecular techniques, including polymerase chain reaction (PCR) and sequencing. Three-layer farms in Bogor, Bekasi, and Cianjur districts, Indonesia, suffered a severe ILT outbreak, and one layer farm in Tangerang district, Indonesia, was declared slightly affected by ILT disease. Clinical and PCR assays were used to diagnose and report all layer farms. There have been reports of this illness in Indonesia, and more recently, outbreaks have been observed in the provinces of Bogor, Bekasi, and Cianjur. The present study used PCR and DNA sequencing methods to examine the ILTV. The work used organs and pooled tracheal swabs from clinically infected and dead chickens to target the envelope gG gene of ILTV. According to the analyses, 15 out of 48 suspicious field samples had isolated positive results. DNA sequencing results revealed that the amplified segment resembled the gene being studied. Information regarding the existence of ILT disease in laying chickens exhibiting respiratory symptoms during the outbreak is provided by this study.