Polymorphism of heat shock protein 70 (Hsp70) gene on local duck of West Sumatera

Abstract

This research aimed to identify the polymorphism of Heat Shock Protein 70 (HSP70) gene on local duck of West Sumatra through PCR-RFLP. The samples used for this research were 45 blood samples of Pitalah ducks (37 females and 8 males) taken at UPT Faculty of Animal Husbandry, Andalas University. Blood samples were isolated with the (G-spinTM Total DNA Extraction Mini Kit protocol) from iNtRON Biotechnology genomic. The isolated DNA was amplified using a pair of primers F: 5'-CGC TGC TGA TTG GCT AGG A-3' and R: 5'-CCT CAC CGC ACG CTT ATT G-3’ target of 1043 bp and F: 5'- TAACACCACCATTCCCACCA-3' and R: 5'-GCGTGGTTACTTTAGAAGAGACA -3' target of 916 bp. The restriction enzymes HaeII step produced genotypes (+/-) 26 samples and (-/-) 19 samples with allele (+) 0,21 and allele (-) 0,79. Restriction enzymes HpyAV was produced genotypes (+/+) 17 samples, (+/-) 26 samples and (-/-) 2 samples with allele (+) 0.67 and allele (-) 0.33. The results of this research indicate that Pitalah duck population in West Sumatera was polymorphic and was in Hardy-Weinberg equilibrium.